BD OptEIA™ 551866 小鼠干扰素-γ ELISPOT (AN-18) set

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Brand:BD OptEIA™

Reactivity:Mouse (QC Testing)

Application:ELISA (Routinely Tested)

Regulatory Status:RUO

RRID:AB_2868943

Description

The OptEIA™ Set for mouse interferon-γ (IFN-γ), utilizing the clone AN-18, contains the components necessary to develop enzymelinked immunosorbent assays (ELISA) for natural or recombinant mouse IFN-γ in serum, plasma, and cell culture supernatants. Sufficient materials are provided to yield approximately 20 plates of 96-wells if the recommended storage, materials, buffer preparation, and assay procedure are followed as specified in this package.

Assay Optimization

BD OptEIA™ Sets allow flexible assay design to fit individual laboratory needs. To design an immunoassay with different sensitivity and dynamic range, the following parameters can be varied: Capture, Detection Antibody titers, Incubation time, Incubation temperature, Assay Diluent formulation, Buffer pH, ionic strength, protein concentration, Type of substrate, Washing technique (i.e., number of wash repetitions and soak times).

Standardization: This immunoassay is calibrated against purified Baculovirus-expressed recombinant mouse IFN-γ.

Preparation And Storage

Store unopened reagents at 2-8°C. do not use reagents after expiration date, or if turbidity is evident. Before use, bring all reagents to room temperature (18-25°C). Immediately after use, return to proper storage conditions. Lyophilized standards are stable until expiration date. After reconstitution, immediately aliquot standard stock in polypropylene vials at 50 µl per vial anf freeze at -80°C for up to 6 months. If necessary, store at 2-8°C for up to 8 hours prior to aliquotting/freezing. Do not leave reconstituted standard at room temperature.

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