PE Goat ALeukocyte Activation Cocktail
Product Information
Material Number: 550583
Size: 200 μL
Description
The most critical step for detection of intracellular accumulations of cytokines by intracellular staining is the isolation and activation of a cell
population to induce production of the cytokine of interest. Without properly activated cytokine-producing cells, cultured in the presence of a
protein transport inhibitor, the ability to detect intracellular accumulations of cytokines in most cases is severely compromised. Various in vitro methods have been reported for stimulating cells to produce cytokines. Polyclonal activators have been particularly useful for inducing cytokine-producing cells. These activators include the following: concanavalin A, lipopolysaccharide, phorbol esters plus calcium ionophore or ionomycin, phytohaemaglutinin, staphylococcus enterotoxin B, and monoclonal antibodies directed against subunits of the TCR/CD3 complex (with or without antibodies directed against costimulatory receptors, such as CD28).
Preparation and Storage
Store product at -80°C prior to use or for long term storage of stock solutions.
Suggested Companion Products
Catalog Number Name Size Clone
554656 Stain Buffer (FBS) 500 mL (none)
Product Notices
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
