sigma D2650二甲基亚砜使用说明

应用DMSO 是一种用于化学反应（如聚合酶链式反应 (PCR)）的极性非质子溶剂，也可作为冷冻保护玻璃化剂用于保存细胞、组织和器官。DMSO 可在细胞冷冻培养基中用于保护细胞免受冰晶导致的机械损伤。它可用于原代、继代培养和重组的异倍体和杂交瘤细胞系、胚胎干细胞 (ESC) 以及造血干细胞的冻存。DMSO 常与 BSA 或胎牛血清 (FBS) 一同使用。包装5mL 和 10mL 火焰封口安瓿瓶包装，100mL 棕色瓶包装其他说明该产品是一种Hybri-Max产品。它经过了杂交瘤测试并对其在细胞冷冻中的适用性进行了评估。该产品经无菌过滤（0.2微米）及内毒素水平检测。注意容易过度冷却，并在室温下缓慢融化固化的产品放在室温下可以再次液化，对其本身无影响。常见问题 What is the molarity of DMSO? DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.  What is the DMSO dissolved in? DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.  Where does DMSO come from? DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.  What concentration of DMSO do I use in a cell freezing media? DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.  Does DMSO have any synthetic utility? DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.  Which document(s) contains shelf-life or expiration date information for a given product? If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.  What is the specification for 'pass' for cell freezing on the Certificate of Analysis for Dimethyl sulfoxide (DMSO)? Product D2438 is assessed for cell freezing.  Cells are frozen and stored at -70°C.  The cells are thawed, cultured and viability is assessed at 24 hours.  There is no evidence of cytotoxicity or abnormal cell growth.  Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid? DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.  What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)? The industry standard for 'endotoxin free' is less than 1 EU/mL.  What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)? DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.  How do I get lot-specific information or a Certificate of Analysis? The lot specific COA document can be found by entering the lot number above under the "Documents" section.  How do I find price and availability? There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.  What is the Department of Transportation shipping information for this product? Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. My question is not addressed here, how can I contact Technical Service for assistance? Ask a Scientist here.