Description
The OptEIA™ Set for mouse interferon-γ (IFN-γ), utilizing the clone AN-18, contains the components necessary to develop enzymelinked immunosorbent assays (ELISA) for natural or recombinant mouse IFN-γ in serum, plasma, and cell culture supernatants. Sufficient materials are provided to yield approximately 20 plates of 96-wells if the recommended storage, materials, buffer preparation, and assay procedure are followed as specified in this package.
Assay Optimization
BD OptEIA™ Sets allow flexible assay design to fit individual laboratory needs. To design an immunoassay with different sensitivity and dynamic range, the following parameters can be varied: Capture, Detection Antibody titers, Incubation time, Incubation temperature, Assay Diluent formulation, Buffer pH, ionic strength, protein concentration, Type of substrate, Washing technique (i.e., number of wash repetitions and soak times).
Standardization: This immunoassay is calibrated against purified Baculovirus-expressed recombinant mouse IFN-γ.