Brand:BD Pharmingen™
Alternative Name:FcγRIII/FcγRII; Fcgr3/Fcgr2
Reactivity:Mouse (QC Testing)
Isotype:Rat SD, also known as Sprague-Dawley (outbred) IgG2b, κ
Immunogen:Mouse BALB/c Macrophage J774
Application:Blocking, Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development), Immunoprecipitation (Reported)
Concentration:0.5 mg/ml
RRID:AB_394656
Storage Buffer:Aqueous buffered solution containing ≤0.09% sodium azide.
Regulatory Status:RUO
准备和存储
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C.
推荐的实验流程
To specifically stain cells bearing FcγII and FcγIII receptors for flow cytometric analysis: Incubate cell suspension with this antibody (≤ 1 μg/million cells) followed by an appropriate fluorochrome-conjugated second-step reagent.
To reduce Fc receptor-mediated binding by antibodies of interest or Fc receptor-mediated binding by PE-CY5 tandem dye conjugates to FcγII and FcγIII receptor-bearing mouse cells for flow cytometric analysis:
1. Preincubate cell suspension with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (eg, ≤ 1 μg/million cells in 100 μl) at 4˚C for 5 minutes.
2. Add antibody of interest directly to preincubated cells in the presence of Mouse BD Fc Block™ (ie, Mouse BD Fc Block™ need not be washed off before staining cells).
3. If anti-Ig second-step is necessary, a reagent must be chosen which will not bind to Mouse BD Fc Block™ (eg, rat IgG2b, κ).
For additional information on using Mouse BD Fc Block™, refer to our website protocol at http://www.bdbiosciences.com/pharmingen/protocols/Immunophenotyping.shtml
