准备和存储
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Brand:BD Pharmingen™
Reactivity:Human (QC Testing), Rhesus (Tested in Development)
Isotype:Human IgG1
Application:Flow cytometry (Routinely Tested)
Concentration:1.0 mg/ml
RRID:AB_2869612
For flow cytometry, incubate 1 million cells suspended in 50-100 µL of staining buffer with 2.5 µg of Human BD Fc Block™ (10 minutes at room temperature) followed by staining with the desired fluorescent antibody. No washing step is needed between the blocking and staining steps.推荐的实验流程